Abstract
Objective To investigate the possibility of transdifferentiation between osteoblasts and adipocytes in rabbits.Methods Adipocytes were isolated from the subcutaneous adipose tissue in the abdomen of 3-month-old New Zealand white rabbits.Then mature adipocytes were cultured and induced to dedifferentiated adipocytes (DA) by ceiling adherent culture method.DA were culutured in osteogenic medium for 3 weeks,and then calcium nodules of the cells were stained by the alizarin red and by immunocytochemical staining for collagen I and alkaline phosphatase (ALP).Osteoblasts were isolated from the skull of one-week-old rabbits by mechanical digestion and enzyme digestion and cultured and amplified in vitro.Then osteoblasta were culutured in adipogenic medium for 3 weeks.Oil Red O staining was used to visualize lipid-rich vacuoles.The expression of peroxisome proliferator activated receptor γ (PPARγ) mRNA was detected by using reverse transcription-polymerase chain reaction (RT-PCR).Results The extracted mature adipocytes turned into fibroblast-like cells (spindle-shaped) after dedifferentiation.After osteogenic induction,the experiment group expressed type Ⅰ collagen,and there was significant difference as compared with control group (P ＜0.05).The ALP activity in experimental group at 3rd,7th and 14th day (0.165± 0.007,0.253 ± 0.005,0.345 ± 0.007 respectively) was higher than that in control group (0.067 ±0.004,0.076 ± 0.006,0.082 ± 0.003 respectively) ( P ＜ 0.05 ).The osteoblasts obtained from the skull were shot-spindle-shaped and positive for Oil Red O staining after culture with adipogenic medium for 3 weeks.The expression of PPARγmRNA in experimental group was positive,that in control group was negative (P ＜0.05).Conclusion Mature adipocytes can turn into DA by in vitroculture.Adipocytes and osteoblasts can differentiate into each other under some particular conditions. Key words: Adipocyte; Dedifferentiation; Osteoblast
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