Rearrangement of the close contact between the mitochondria and the sarcoplasmic reticulum in airway smooth muscle

Abstract

The mitochondria and the sarcoplasmic reticulum (SR) are two major intracellular calcium-storing organelles that exhibit close functional interaction with each other. Close spatial association is believed to be important for their functional interaction. In this study, we have characterized the spatial relationship between the SR and the mitochondria in porcine tracheal smooth muscle cells (TSMC) under different conditions. By examining the cross-section of unstimulated TSMC with electron microscopy, we found that 99.4 ± 0.5% of the mitochondria seen on random cross-sections were situated within 30 nm of the SR and that 82.2 ± 6.7% of the mitochondria were completely enveloped by the SR network. Overall, 48.0 ± 3.5% of the mitochondrial outer membrane was within 30 nm with the SR. After stimulation of the TSMC with acetylcholine (ACh) or 80 mM [K +] solution 97.0 ± 2.1% and 98.6 ± 1.4% of the mitochondria observed were situated within 30 nm of the SR, respectively. However, the proportion of the mitochondria that was completely enveloped by the SR was significantly reduced to 12.2 ± 5.9% in ACh-stimulated cells and 9.7 ± 6.6% in 80 mM [K +] stimulated cells. The percentage of mitochondrial membrane closely associated with the SR was correspondingly lower at 10.1 ± 1.0% during ACh stimulation and 10.8 ± 0.9% during 80 mM [K +] stimulation. During smooth muscle cell stimulation, the SR appears to unwrap from the mitochondria and extend into the cytoplasm while maintaining close contact with the mitochondria over a smaller area. Such static and dynamic components of the close spatial association between the mitochondria and the SR may serve as a structural basis for the selective and efficient Ca 2+ trafficking between the two organelles in TSMC.