Rearrangement and morphological transition of DNA on adsorbent templates of hydrocarbon/fluorocarbon phase‐separated monolayers

Abstract

AbstractA phase‐separated template prepared from a mixed monolayer of fluorinated long‐chain s‐triazine derivatives and comb copolymers containing s‐triazine rings was morphologically controlled by DNA adsorption. The adsorbed morphology of DNA was formed through a morphological transition process at the air/water interface rather than by maintaining the shape of the phase‐separated template. Dot‐like domains, circular domains, centrally protruding domains, network morphologies, and even co‐continuous surface morphologies of DNA were obtained. The phase‐separated monolayer used as a template depends on the mixed ratio of the fluorocarbon and hydrocarbon copolymer components, the copolymerization ratio of the hydrocarbon copolymers, and the chain length of the fluorinated long‐chain s‐triazine derivative, and various morphological transitions occur. The DNA introduced into the sub‐phase was selectively adsorbed onto the s‐triazine ring‐containing comb copolymer, and the adsorbed DNA assembly exhibited a morphological transition based on concerted interactions. Furthermore, by utilizing a fluorescent probe molecule, evidence of DNA morphogenesis itself was supported by its emission/quenching behavior.Highlights Morphological control of the DNA assembly was attained. Immobilization of DNA assemblies on phase‐separated templates was achieved. Preparation of patterned templates was realized by copolymers a fluorinated derivative. Adsorption of DNA to the diamino‐s‐triazine ring was achieved by hydrogen bonding. A rearrangement of the DNA assemblies on the template was achieved.