Abstract
We developed a near-infrared ratiometric fluorescent nanoprobe with single-excitation dual-emission. The proposed nanoprobe was applied for real-time monitoring the changes in the pH value during hydrogen peroxide-induced apoptosis by ratio imaging. The pH value of HeLa cells changed from 6.6 to 5.6 after H2O2 (100 μM) stimulation for 40 min.
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