Real-time Polymerase Chain Reaction To Diagnose Lymphogranuloma Venereum

Servaas A Morré,Johannes S.A Fennema,Roel A Coutinho,Joke Spaargaren,A Salvador Peña,Henry J.C De Vries

doi:10.3201/eid1108.050535

Abstract

Real-time Polymerase Chain Reaction To Diagnose Lymphogranuloma Venereum

Highlights

Ing primers and probes were selected: LGV-F 5′ CTG TGC CAA CCT CAT CAT CAA 3′, LGV-R 5′ AGA CCC TTT CCG AGC ATC ACT 3′, and LGV MGB-probe 6-FAM-CCT GCT CCA ACA GT
LGV strains L1, L2, L2b, and L3 tested positive in both the TaqMan and Rotorgene assays, which shows the analytical specificity of real-time polymerase chain reaction (PCR)
We determined in a blinded setting the presence of LGV in a selected group of patients according to C. trachomatis–positive rectal swab (Chlamydia 2SP Collection & Transport Kit [Quelab] by commercially available PCR (COBAS AMPLICOR, Hoffman-La Roche Ltd)

Summary

Introduction

Ing primers and probes were selected: LGV-F 5′ CTG TGC CAA CCT CAT CAT CAA 3′, LGV-R 5′ AGA CCC TTT CCG AGC ATC ACT 3′, and LGV MGB-probe 6-FAM-CCT GCT CCA ACA GT. We tested different C. trachomatis serovars and serovariants A, B, Ba, C, D, Da, D-, E, F, G, Ga, H, I, Ia, I-, J, Jv, K, L1, L2, L2b, L3, C. muridarum (MoPn), C. pneumoniae, C. pecorum, C. psittaci, and 32 other microorganisms that normally reside in the human perianal and urogenital region and in the oropharynx.

Results

Conclusion