Abstract
I read with interest the article by Joseph and associates evaluating the efficacy of quantitative real-time polymerase chain reaction (qPCR) in the diagnosis of postoperative bacterial endophthalmitis after cataract surgery. 1 Joseph C.R. Lalitha P. Sivaraman K.R. Ramasamy K. Behera U.C. Real-time polymerase chain reaction in the diagnosis of acute postoperative endophthalmitis. Am J Ophthalmol. 2012; 153: 1031-1037 Abstract Full Text Full Text PDF PubMed Scopus (33) Google Scholar This technique offers a rapid pathogen identification and may be very useful in the treatment of postoperative endophthalmitis, in which the type of organism plays an important role in the final visual outcome. 2 Endophthalmitis Vitrectomy Study GroupResults of the Endophthalmitis Vitrectomy Study: a randomized trial of immediate vitrectomy and of intravenous antibiotics for the treatment of postoperative bacterial endophthalmitis. Arch Ophthalmol. 1995; 113: 1479-1496 Crossref PubMed Scopus (1254) Google Scholar , 3 Lalwani G.A. Flynn Jr, H.W. Scott I.U. et al. Acute-onset endophthalmitis after clear corneal cataract surgery (1996–2005) Clinical features, causative organisms, and visual acuity outcomes. Ophthalmology. 2008; 115: 473-476 Abstract Full Text Full Text PDF PubMed Scopus (149) Google Scholar , 4 Pijl B.J. Theelen T. Tilanus M.A.D. Rentenaar R. Crama N. Acute endophthalmitis after cataract surgery: 250 consecutive cases treated at a tertiary referral center in the Netherlands. Am J Ophthalmol. 2010; 149: 482-487 Abstract Full Text Full Text PDF PubMed Scopus (76) Google Scholar Their conclusions, however, should be interpreted with care. Real-time Polymerase Chain Reaction in the Diagnosis of Acute Postoperative EndophthalmitisAmerican Journal of OphthalmologyVol. 153Issue 6PreviewTo evaluate the efficacy of quantitative real-time polymerase chain reaction (qPCR) in the diagnosis of postoperative bacterial endophthalmitis among patients who underwent cataract surgery at a tertiary care center. Full-Text PDF ReplyAmerican Journal of OphthalmologyVol. 154Issue 6PreviewWe appreciate Dr Crama's comments regarding our recent study of the use of quantitative real-time polymerase chain reaction (qPCR) in the diagnosis and management of postoperative endophthalmitis.1 Dr Crama raises several important points that we address herein. Full-Text PDF
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