Abstract

ABSTRACT: Rapid detection of pathogenic and spoilage microorganisms is essential for ensuring the safety and quality of food. Real-time polymerase chain reaction (PCR) technology has the potential to achieve rapid, sensitive, and specific detection of these microorganisms in food. In this review, we discuss real-time PCR technologies in use today, applications of real-time PCR in food systems, and some of the associated challenges and limitations.

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