Real-time PCR forFrancisella tularensisTypes A and B

Kiersten Kugeler,Ryan Pappert,Jeannine Petersen,Yan Zhou

doi:10.3201/eid1211.060629

Abstract

Real-time PCR for<i>Francisella tularensis</i>Types A and B

Highlights

Pradines B, Hovette P, Fusai T, Atanda HL, Baret E, Cheval P, et al Prevalence of in vitro resistance to eleven standard or new antimalarial drugs among Plasmodium falciparum isolates from Pointe-Noire, Republic of the Congo
We developed real-time TaqMan PCR assays for classification of F. tularensis type A and type B after F. tularensis is identified by culture or, in the absence of culture, by a PCR method such as the F. tularensis multitarget TaqMan assay [5]
To evaluate the ability of the type A and type B TaqMan assays, in conjunction with the multitarget assay, to identify F. tularensis and classify subspecies in primary specimens, human, animal, and tick samples were tested (Table) available from DNA was extracted from 200 μL fluid, 25 mg liver, and 10 mg spleen or lung by using the QIAamp DNA MiniKit (Qiagen, Valencia, CA, USA) and 1 μL tested

Summary

Introduction

Pradines B, Hovette P, Fusai T, Atanda HL, Baret E, Cheval P, et al Prevalence of in vitro resistance to eleven standard or new antimalarial drugs among Plasmodium falciparum isolates from Pointe-Noire, Republic of the Congo. We developed real-time TaqMan PCR assays for classification of F. tularensis type A and type B after F. tularensis is identified by culture or, in the absence of culture, by a PCR method such as the F. tularensis multitarget TaqMan assay [5]. Assays were optimized by using 1 ng of type A (strain SchuS4) or type B (strain LVS) DNA on the LightCycler 1.2 (Roche Applied Science, Indianapolis, IN, USA).

Results

Conclusion