Real-Time Monitoring of Stem Cells by Diamond-Based Impedance Sensors

Václav Procházka,Roman Matějka,Lucie Bačáková,Tibor Ižák,Jana Štěpanovská,Alexander Kromka,Ondrej Szabó

doi:10.3390/proceedings1040515

Abstract

Cell-based impedance spectroscopy is a promising label-free method for electrical monitoring of cell activity. Here we present a diamond-based impedance sensor with built-in gold interdigitated electrodes (IDEs) as a promising platform for simultaneous electrical and optical monitoring of adipose-derived stem cells (ASC). The impedance spectra were collected in a wide frequency range (from 100 Hz to 50 kHz) for 27 h of cultivation in chambers designed for static cultivation. Absolute impedance spectra were analyzed in terms of measurement frequencies and cell properties monitored by high-resolution digital camera.

Highlights

Bioelectrical sensors are of high interest due to non-invasive label-free techniques for in vitro monitoring of biological events and due to simplicity and fast response in real time
The brighter regions represent the diamond film grown on Au interdigitated electrodes (IDEs) and darker regions correspond to the film grown on the quartz substrate
The diamond character of the deposited film was confirmed by Raman spectra measured at two different positions: on a quartz substrate and Au layer

Summary

Introduction

Bioelectrical sensors are of high interest due to non-invasive label-free techniques for in vitro monitoring of biological events and due to simplicity and fast response in real time. There are two analytic approaches which employ either optical or electronic signal processing [1]. Both these approaches reached state of the art in a transducer type which converts a stimulus-induced cellular response into the quantifiable signal (i.e., biosensor signal). We have already introduced an optically transparent surface-conductive diamond thin film as a functional layer in impedance sensors for biological studies [4] and recognition of gas and chemical molecules [5,6]. An impedance sensor with the functional diamond layer deposited on gold interdigitated electrodes is employed to electrically and optically monitor the activity of adipose-derived stem cells

Methods

Results

Conclusion