Real-Time Human and Guinea Pig Action Potentials Anthropomorphized from Neonatal Mouse Cardiomyocytes

Abstract

The murine cardiac action potential waveform can be anthropomorphized into that of a human-like waveform in real time, through a novel dynamic-clamp method known as the cell-type transforming clamp (CTC). In the CTC, a computationally calculated virtual conductance is inserted into the cell in real time, to compensate for the differences between murine and human sarcolemmal currents. By so doing, the CTC anthropomorphizes the membrane potential without clamping it, thereby enabling the investigation of drug- or mutation-induced arrhythmogenic phenotypes in the appropriate human action potential context (but in the experimentally powerful mouse animal model).We are using a real-time implementation of a genetic algorithm that optimizes the morphology of a theoretical model, in order to match the murine action potential recorded from a real cell. We present a comparison of human and guinea pig action potentials anthropomorphized in real time, from neonatal mouse cardiomyocytes.