Abstract
A biopsy is often performed for the diagnosis of cancer during a surgical operation. In addition, pathological biopsy is required to discriminate the margin between cancer tissues and normal tissues in surgical specimens. In this study, we presented a novel method for discriminating between tumor and normal tissues using fluorescence lifetime endoscopy (FLE). We demonstrated the relationship between the fluorescence lifetime and pH in fluorescein using the proposed fluorescence lifetime measurement system. We also showed that cancer could be diagnosed based on this relationship by assessing differences in pH based fluorescence lifetime between cancer and normal tissues using two different types of tumor such as breast tumors (MDA-MB-361) and skin tumors (A375), where cancer tissues have ranged in pH from 4.5 to 7.0 and normal tissues have ranged in pH from 7.0 to 7.4. To support this approach, we performed hematoxylin and eosin (H&E) staining test of normal and cancer tissues within a certain area. From these results, we showed the ability to diagnose a cancer using FLE technique, which were consistent with the diagnosis of a cancer with H&E staining test. In summary, the proposed pH-based FLE technique could provide a real time, in vivo, and in-situ clinical diagnostic method for the cancer surgical and could be presented as an alternative to biopsy procedures.
Highlights
A biopsy is often performed for the diagnosis of cancer during a surgical operation
The basic configuration of the proposed system is the combination of the real time analog mean delay (AMD)-fluorescence lifetime microscopy (FLM) and optical endoscopy
In order to elucidate difference in fluorescence lifetime for normal and tumor tissues displayed in Fig. 4, we evaluated it between normal tissues (N = 20) and tumor tissues with MDA-MB-361 breast cancer (N = 10) and A375 skin cancer (N = 10) using ANOVA statistical analysis by PRISM 9.1.0.221 (GraphPad Software Inc., https://www.graphpad.com/scientific-software/prism/)
Summary
A biopsy is often performed for the diagnosis of cancer during a surgical operation. In addition, pathological biopsy is required to discriminate the margin between cancer tissues and normal tissues in surgical specimens. To facilitate the diagnosis of cancer during a surgical operation, biopsy is generally performed by a surgeon to determine the presence of tumor tissues by using hematoxylin and eosin (H&E) staining under an optical microscopy by a pathologist. A cancer in gastroenterology with confocal laser endoscopy system that allows the minimally invasive discrimination of an abnormal lesion on a tissue surface in vivo with high resolution cell imaging[14,15] These clinical approaches have enabled surgeons to immediately determine the histopathological evaluation and the diagnosis of a tumor without biopsy during a surgical operation
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
