Abstract
Abnormal variations of lysosomal pH values are closely associated with various diseases, thus it is of great necessity to develop some reliable lysosome-targeted fluorescent probes for real-time monitoring pH changes. Herein, a pyridine-decorated ratiometric fluorescent probe Lyso-CMCP based on camphor-coupled coumarin fluorophore was developed for monitoring pH fluctuations in lysosome of living cells. Lyso-CMCP could sensitively and selectively respond to acidic pH in the range of 7.54–3.19 with a significantly red-shifted fluorescence emission from 455 nm to 516 nm. Besides, this probe also possessed some favorable features, such as good photostability, fine reversibility, excellent anti-interference ability, and low cytotoxicity. The pKa value of 5.14 endowed Lyso-CMCP with the potential to accumulate in lysosomes. With this probe in hand, the lysosomal pH fluctuations were successfully monitored and quantified by ratiometric fluorescence imaging.
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