Real-time tracking of endogenous CYP2J2 by red-emitting aggregation-induced emission probe with large Stokes shift

Abstract

The abnormal expression of cytochrome P450 2J2 (CYP2J2) is closely related to the proliferation of human cancer cells. In this study, a red-emitting probe with an aggregation-induced emission (AIE) effect for the quantification of CYP2J2 was developed by modifying the typical AIE fluorescent group tetraphenylethylene (TPE) through two-step synthesis. Upon addition of CYP2J2, the probe underwent O-demethylation and spontaneously 1,6-elimination reactions of p-hydroxybenzyl that allowed it to alter its photophysical properties, thus realizing light-up detection of CYP2J2 with extremely large Stokes shift (236 nm), excellent sensitivity and selectivity, low detection limit (1.09 nM). Moreover, this new AIE-active probe with good biocompatibility was admirably employed to tracking endogenous CYP2J2 in living cells.