Abstract
A new planar surface plasmon resonance sensor with spectral interrogation was constructed and tested. The SPR device was directly compared with a high-end commercial BIAcore 2000 instrument. In immunological applications, we used the SPR-device for the detection of myoglobin and to measure the concentration of staphylokinase in a competitive assay. The lower limits of detection were tested when the binding of Ni 2+-ions on a matrix with nitrilotriacetic acid (NTA) was measured. The tested sensor was comparable in performance to the commercial instrument. Its detection of the plasmon resonance in a spectral mode and its fiber optical connection between sensor and detector unit offer additional advantages for future applications in on-site analysis with a portable detector.
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