Abstract
AbstractTraditional PCR (Polymerase Chain Reaction) technology is based on an end‐point system which confirms the existence of a target DNA sequence by electrophoresis and UV transillumination steps. This process is labor‐intensive and time consuming. A real‐time PCR is the best alternative that can improve problems of analysis time and accuracy. Therefore, we propose a smaller, non‐optical and non‐labeling system with the advantages of an established real‐time PCR. The proposed technique utilizes a variation of the thermal conductivity of the DNA sample with a PCR cycle. We confirmed that thermal conductivity depends on amounts of PCR products.
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