Real Time Pcr Detection Of Aspergllus Fumigatus, Aspergillus Flavus And Aspergillus Niger Based On Polyketide Synthase Gene

Abstract

FEBRUARY 2012 AB6 Abstracts S A T U R D A Y 21 Cord Blood Cellular Proliferative Response as a Predictive Factor For Atopic Dermatitis At 6 Months of Age H. Kim, J. Seo, H. Kim, J. Kwon, B. Kim, J. Shim, W. Kim, D. Song, S. Lee, S. Lee, G. Jang, K. Kim, K. Ahn, Y. Shin, J. Yu, M. Kang, K. Lee, S. Hong; Inje University Sanggye Paik Hospital, Seoul, REPUBLIC OF KOREA, University of Ulsan College of Medicine, Seoul, REPUBLIC OF KOREA, Seoul National University Bundang Hospital, Seongnam, REPUBLIC OF KOREA, Inje University Haeundae Paik Hospital, Pusan, REPUBLIC OF KOREA, Sungkyunkwan University School of Medicine, Seoul, REPUBLIC OF KOREA, Inje University Seoul Paik Hospital, Seoul, REPUBLIC OF KOREA, College of Medicine, Korea University, Seoul, REPUBLIC OF KOREA, Hallym University College of Medicine, Seoul, REPUBLIC OF KOREA, Ajou University School of Medicine, Suwon, REPUBLIC OF KOREA, National Health Insurance Corporation Ilsan Hospital, Ilsan, REPUBLIC OF KOREA, Yonsei University, Seoul, REPUBLIC OF KOREA, CHA University School of Medicine, Seoul, REPUBLIC OF KOREA. RATIONALE: When a person exposed to allergens earlier as fetal period, the risk to develop allergic diseases increase. Therefore immunologic status of fetus may be associated with the development of allergic disease. We evaluated the factors that could have an effect on the development of atopic dermatitis (AD) at 6 months with cord blood mononuclear cells (CBMCs). METHODS: We constructed Cohort for Childhood Origin of Asthma and Allergic Diseases (COCOA) in general population from 2007 and diagnosed AD by allergy specialists in 371 infants. Cord blood was collected from them and measured cell count and total IgE. When CBMCs were extracted, ovalbumin (OVA) 100 ug/mL and phytohaemagglutinin (PHA) 10 ug/mL were stimulated and cellular proliferative response (CPR) and cytokine levels (IL-13 and IFN-g) were measured. RESULTS: The risk to develop AD at 6 months is increased in boys (OR 3.04, 95% CI 1.64-5.65) and when maternal education level was high (OR 1.97, 95% CI 1.07-3.61). Parental history of AD increased the risk of AD diagnosis (OR 2.39, 95% CI 1.18-4.81). With PHA stimulation, CPR was increased in AD-diagnosed group compared to infants without AD (P50.013), but with OVA stimulation, there was no difference between the two groups (P50.180). In addition, there was no significant difference in IFN-g/IL-13 ratio to PHA stimulation. CONCLUSIONS: The independent risk factors for the development of AD at 6 months were male, high maternal education level, and parental history of AD. Increased CPR with PHA stimulation may predict the development of AD at 6 months.