REAL TIME PCR BASED A PILOT STUDY IN INDIAN PATIENTS ON ENDOMETRIAL GENE EXPRESSION

Abstract

Aim and Objective: To evaluate the role of important endometrial receptivity genes in females under Assisted Reproductive Technology (ART) Background: Receptivity concept remains still an undeniable question for the success of embryo implantation. Diagnostic and prognostic tools available are not completely reliable as well as are very expensive, adding overall cost to the Assisted Reproductive Technology (ART). A lot of research is underway for better understanding the basics of the endometrial implantation. Materials and methods: A total of 27 female endometrial samples were used for the study. 5 were control (women under ART treatment and had successful pregnancy outcome) and 22 experimental samples of females undergoing ART treatment. These samples were further processed for gene expression profiling of 6 selected genes (CTNNA2, CXCL14, GADD45A, LIF, SFRP4 and TIMP3) by qRT-PCR. Fold change were calculated for all 22 samples which were compared with control as well as earlier studies and diversion from earlier studies were noted. Results: qRT-PCR results of all 22 experimental samples with in-house control did not behave same as earlier studies detecting receptivity expression profile. According to Diaz-gimeno et al 2011, of 6 selected genes, 2 genes (CTNNA2, SFRP4) and 4 genes (CXCL14, GADD45A, LIF & TIMP3) were believed to be downregulated and upregulated respectively as predictors of receptive endometrial. None of our samples exactly matched this description. Especially SFRP4 gene had opposite expression levels in most of the samples. Conclusion : There is varying difference of gene expression among the samples compared to earlier studies. Hence, systematically scanning of important genes can help in determining receptivity criteria in detail. Eventually cost effective testing method can be developed.