Abstract
Interleukin-6 (IL-6) is a central protein in the regulation of the inflammatory and immunologic response (1). A G→C polymorphism at position −174 has been associated with osteoporosis (2), juvenile rheumatoid arthritis (3), and atherosclerosis (4) with increased risks in the absence of the CC genotype. Restriction fragment length polymorphism analysis has been used for IL-6 genotyping, but it is time-consuming and requires multiple manual steps. To improve throughput, we developed a rapid-cycle PCR method on the LightCyclerTM instrument (Roche Diagnostics) with fluorescent probe melting analysis. This assay is completed in 60 min. DNA from 115 German Caucasians was extracted from whole blood according to standard procedures. …
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