Abstract
Reduction of Complex I (NADH:ubiquinone oxidoreductase I) from Escherichia coli by NADH was investigated optically by means of an ultrafast stopped-flow approach. A locally designed microfluidic stopped-flow apparatus with a low volume (0.2μl) but a long optical path (10mm) cuvette allowed measurements in the time range from 270μs to seconds. The data acquisition system collected spectra in the visible range every 50μs. Analysis of the obtained time-resolved spectral changes upon the reaction of Complex I with NADH revealed three kinetic components with characteristic times of <270μs, 0.45–0.9ms and 3–6ms, reflecting reduction of different FeS clusters and FMN. The rate of the major (τ=0.45–0.9ms) component was slower than predicted by electron transfer theory for the reduction of all FeS clusters in the intraprotein redox chain. This delay of the reaction was explained by retention of NAD+ in the catalytic site. The fast optical changes in the time range of 0.27–1.5ms were not altered significantly in the presence of 10-fold excess of NAD+ over NADH. The data obtained on the NuoF E95Q variant of Complex I shows that the single amino acid replacement in the catalytic site caused a strong decrease of NADH binding and/or the hydride transfer from bound NADH to FMN.
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