Abstract
Because of their high magnetic susceptibility, superparamagnetic iron oxide nanoparticles (SPIONs) have shown potential applications in various fields. However, their application in optical imaging was severely restricted because of the lack of fluorescence in them. Here, we present a real-time in situ giant fluorescence enhancement, at least an order of magnitude higher in self-assembled SPIONs in comparison to a single SPION at the single-particle level under the applied magnetic field (MF). The single-particle tracking video provides the direct observation of the self-assembly and disassembly and the associated fluorescence change in SPIONs in the presence and absence of the applied MF. A theoretical model was applied to calculate the dipole–dipole interaction energy, drag coefficient, and quantified the dynamics of this aggregation.
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