Abstract
Rapid methods for assessing engineered nanoparticle (NP) and their interactions with lipid membranes is important to several fields including pharmaceutical, clinical and toxicological studies. This study presents Resonance Enhanced Surface Impedance spectroscopy (RESI) as a method for real time assessment of NP interactions. RESI, used in a flow-injection analysis mode, provides a rapid and versatile method for revealing lipid disruption and reorganisation. Ferrocenated gold NPs (FcHT-AuNP) of three different sizes, were used to study their interactions with supported lipid layers. Electrochemistry and Atomic Force Microscopy (AFM) was used to complement RESI results, showing, that with increased incubation, AuNPs tend to agglomerate on the substrate. The effect of the change in hydrophilicity of the FcHT-AuNP upon oxidation, was immediately evident, using RESI, giving direct evidence that in its ferrocenium form the NPs tend to resist lipid adsorption.
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