Real-time monitoring of immunochemical interactions with a tantalum capacitance flow-through cell

Abstract

A flow-through cell for the real-time capacitance monitoring of immunochemical interactions has been developped. It consists of a tantalum strip onto which tantalum oxide was grown electrochemically to a layer thickness as small as 5 nm. Antibody or antigen was immobilized onto the tantalum oxide surface, and binding of the corresponding analyte resulted in modification of the electrical capacitance of the system. With mouse IgG as the ligand, real-time monitoring of anti-mouse-IgG inthe nanogram per milliliter range was possible