Abstract
Callistemon citrinus is a member of Myrtaceae family that thrives under different ecological conditions. The leaves, flowers, stem backs and roots of the plant contain various phytochemicals that are useful in folk medicine for different remedies such as antimicrobial, anti-nociceptive, fungicide and anti-inflammatory purposes. In this study, we investigated the cytotoxic effect of Callistemon citrinus leaf and flower methanolic extracts against human Colo-205 Cell Line using real time cell analyzer device for monitoring in time-dependent manner. To determine the mechanism of cytotoxicity of the extracts, Western blotting assay was used for measuring evocation of Akt pathway. Extracts were found to exert cytotoxic effect at a dose dependent manner. IC50 values of leaves and flowers extract were 6.49 µg/mL and 5.22 µg/mL, respectively. At the early stages of the experiment, Akt pathway was triggered at high extract concentrations. Although, high extract concentrations showed proliferative effect at early stages, this effect reversed after 5 and 8 h resulting in low cell viability. Findings from this study therefore showed that extracts of leaf and flower from Callistemon citrinus demonstrated cytotoxic effect against Colo-205 but seems not to be related Akt signaling pathway.
Highlights
Colon cancer is a one of the major causes of mortality and morbidity all over the World
The aim of this study was investigate the dynamic monitoring of cytotoxic effect of citrinus species’ flower (CF) and CL extract on Colo-205 cell line
Cell culture Colo-205 cell line was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) The cells were grown in DMEM with 10% fetal bovine serum, 2 mM L-glutamine and 1% penicillin/streptomycin and incubated in a humidified at
Summary
Colon cancer is a one of the major causes of mortality and morbidity all over the World. In addition to conventional treatment of colon carcinoma, there are a lot of alternative medicine in use from natural products. One of such natural products include phytochemicals from ornamental plants. Callistemon citrinus is one of the medicinal ornamental plants from Myrtaceae family (Brophy et al 1998). The in vitro cytotoxic effects of leaf and flower extracts from C.citrinus has recently been reported against MCF-7 breast cancer cell line by our group (Fayemi et al 2015)
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