Abstract
Cytokine secretion researches have been a main focus of studies among the scientists in the recent decades for its outstanding contribution to clinical diagnostics. Localized surface plasmon resonance (LSPR) technology is one of the conventional methods utilized to analyze these issues, as it could provide fast, label-free and real-time monitoring of biomolecule binding events. However, numerous LSPR-based biosensors in the past are usually utilized to monitor the average performance of cell groups rather than single cells. Meanwhile, the complicated sensor structures will lead to the fabrication and economic budget problems. Thus, in this paper, we report a simple synergistic integration of the cell trapping of microwell chip and gold-capped nanopillar-structured cyclo-olefin-polymer (COP) film for single cell level Interleukin 6 (IL-6) detection. Here, in-situ cytokine secreted from the trapped cell can be directly observed and analyzed through the peak red-shift in the transmittance spectrum. The fabricated device also shows the potential to conduct the real-time monitoring which would greatly help us identify the viability and biological variation of the tested single cell.
Highlights
Cytokines are a broad and loose category of small immunological protein biomarkers secreted by the immune cells
The anodization conditions have already been optimized for ease of removing the nanoimprinted COP film from the mold and formation of the nanostructures
As the diameter of target Jurkat cells were ranging from 8–12 μm, the Msiicnrgomleaccheinlleso2c0c2u0,p1a1,n1c0y7 efficiency became relatively low when the trapping well diameter was less4 othf 1a0n 10 μm or over 16 μm which would lead to a high possibility to trap none or multiple cells
Summary
Cytokines are a broad and loose category of small immunological protein biomarkers secreted by the immune cells. They play a critical role in adjusting the cell signaling, cell differentiation and biological response in the human immune system, and are proven to be involved in cell autocrine, paracrine and endocrine signaling as immune-modulating agents [1,2,3,4]. The IL-6 is responsible for stimulating acute phase protein synthesis, as well as the production of neutrophils in the bone marrow. It supports the growth of B cells and is antagonistic to regulatory T cells [6,7,8]. The detection of IL-6 becomes our first target in this research
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