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Abstract
Clathrin mediated endocytosis is a crucial factor in maintaining cellular dynamics. So far, real-time observation of this phenomenon has been achieved using methods that are limited to two dimensional spatial analysis even though cells have three dimensional geometries. Here we have used a fast, confocal based, three dimensional automated tracking scheme to analyze clathrin coat dynamics at the ventral and dorsal surfaces of mammalian cells. This technique presents a direct observation of a clathrin mediated virus entry at the apical surface of polarized cells for the first time.
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