Abstract
Small tetracysteine insertions are more suitable for fluorescence resonance energy transfer (FRET) studies of protein folding and small complex assembly than bulky GFP-based fluorophores. Here, we describe a procedure for expression, purification, and fluorescent labeling of a FRET-based probe, called CSNAC that can track the conformational changes undergone by SNAP-25 as it folds in the exocytic complex. The fluorescent protein Cerulean was attached to the N-terminus and served as a FRET donor. The biarsenical dye FlAsH, served as a FRET acceptor, was bound to a short tetracysteine motif positioned in the linker domain of SNAP-25. CSNAC can report real-time FRET changes when the Syntaxin soluble domain is added in vitro.
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