Abstract

Rapid quantification of plasma ferritin levels holds utmost importance for the effective management of different chronic illnesses. We report the development of a novel biosensor for quantitative and selective detection of ferritin from a drop of blood plasma. Developed electrochemical biosensing platform contains a semiconductor nano-structured decorated screen-printed electrode (SND-SPE). The hydrothermally synthesized ZnO-Mn3O4 nanocomposite which has been coated on the electrode surfaces, imparts the specificity in ferritin diagnostics. Cyclic voltametric (CV) measurements with blood plasma shows a prominent reduction peak of ∼ - 0.76 V for specific ferritin reduction. The amperometric sensor shows a known concentration of 0.3 µg/dl ferritin-containing plasma generates 15 µA of current for single-time use. The efficacy of the device is evaluated by detecting ferritin in human plasma samples. The limit of detection and response time of the developed sensor are 0.04 µg/dl and 0.1 s respectively. The layer of ZnO-Mn3O4 nanocomposite has played as an excellent catalyst during the specific reduction of Fe3+ ion and the merits of the device in terms of high robustness, ultrafast detection, highly stable, low-cost, and a biodegradable sensor, make it attractive for the deployment in point-of-care settings.

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