Abstract
A new confocal microscope has several unique features which differentiates it from other in vivo confocal imaging systems.is described. • The light source is a halogen lamp. This source has many advantages over the mercury or xenon arc lamps used in other confocal designs. The mercury or xenon arc lamps have the problem of arc jitter which results in changing illumination intensity. Filters are inserted in the lamp housing to remove both short ultraviolet and infrared light. • The microscope uses standard microscope objectives which are readily interchangeable. Other in vivo confocal systems are limited to a built in objective which is not removable. In these studies we used a Leitz 50X, NA 1.0 water immersion objective.• The detection system consists of an intensitified video camera with video output to a Sony U-matic tape recorder. In parallel with the video recording there is a video monitor in order that the operator can observe in real-time the confocal images of the subject's eye.
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