Abstract
Proteins have to be flexible enough to support turn-over rates up to hundreds per second, yet stable enough to maintain their three-dimensional structure over hours and days. As result of thermal excitation they fluctuate between structural conformations. We measured thermally excited structural fluctuations in the Adenylate Kinase using a site-specifically attached nanoparticle and a laser trap based position sensing scheme. This ‘Thermal Noise Imaging’ can provide real-time tracking of 3D structural transitions. We present details of the technique and a comparison of thermally excited structure fluctuations with functional transitions.
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