Abstract
We have constructed a reagentless lactate sensor using lactate oxidase (LOD) covalently attached to an electropolymerized copolymer film, poly(5-hydroxy-1,4-naphthoquinone- co-5-hydroxy-3-thioacetic acid-1,4-naphthoquinone), poly(JUG- co-JUGA). Around 10 −12 M cm −2 of covalently bound enzymes are immobilized on these films. In aerated medium, the amperometric response versus lactate concentration shows a sensitivity of 350±50 μA M −1 cm −2 for an applied potential of +0.5 V versus Ag|AgCl on a film-coated Pt electrode. In deaerated medium, the quinone group, conjugated with the polymer backbone, acts as an immobilized mediator. An amperometric response is observed on film-coated glassy carbon (GC) electrode at a potential as low as −0.1 V versus Ag|AgCl, with a sensitivity of 110±40 μA M −1 cm −2.
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