Abstract
Monoclonal antibodies to proteins encoded by the ras, myb, myc, erb-B, src and PDGF-2 genes were tested for reactivity with normal rat liver, livers from rats fed with 0.06% 2-acetylaminofluorene (AAF), and premalignant lesions and primary liver tumours from rats given AAF alone or a combined treatment with diethylnitrosamine and AAF. Radioimmunoassays were performed with plasma membrane fractions and total soluble subcellular extracts of the tissues, and immunoperoxidase staining was carried out on frozen tissue sections. All of the antibodies were positive in radioimmunoassays, some more strongly than others, and each antibody bound equally to extracts of different kinds of tissue. Immunohistology revealed significant staining of normal liver by 5 of the 6 antibodies, and only minor qualitative differences of the staining pattern in some tumours and hyperplastic nodules. It was concluded that these antibodies were not able to discriminate sufficiently well between normal, premalignant and malignant rat liver to be of value in identifying the precursor cells of malignant tumours.
Highlights
Primary tumours developed from 7 months onwards
The highest activity was seen with anti-ras antibody, but it is not possible to draw firm conclusions about the relative concentrations of the different oncoproteins in a given tissue extract because the antibody content of the ascites fluid was not precisely known, or the affinities of the antibodies
The principal finding in this experiment was that there was no significant difference in binding of any of the antibodies to extracts of normal liver, liver from AAF-treated rats, or primary AAF-induced liver tumours
Summary
The purpose of the present study was to determine the reactivity of a panel of such monoclonal antibodies with normal, pre-malignant and malignant liver tissues derived from an experimental rat model in which a variety of identifiable lesions are induced in response to carcinogen treatment
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