Abstract
It was studied whether the microcytotoxicity test of simple manual method was useful or not to examine the reactivity of monoclonal antibodies (OKT3, T4, T8, T11, M1, Ia1) on human mononuclear cells (MNC). The test materials were MNC of 39 healthy donors and those of 16 patients, and their T and B cells isolated by the column method. Record results of % cell lysis were judged by the 10 grades scale.The results of normal MNC were coincident with those of the other manual methods and the flow cytometry. Our test method was confirmed to be useful also to T and B cells isolated by the column method. In case of abnormal MNC, the results of chronic renal failure and adult T cell leukemia were coincident with those of previous reports. As the result, this method could be put to practical use.
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