Reactive Oxygen Species of Gastric Antral Mucosa in Patients with Peptic Ulcer Disease

Abstract

Background and Objective: Researchers now have a better understanding of the mechanisms involving Helicobacter pylori (H. pylori) infection in peptic ulceration. H. pylori is considered to play a critical role in duodenal ulcers (DU) as well as gastric ulcers (GU). It also plays a key role in the production of reactive oxygen species (ROS) in gastric mucosa. However, the role of gastric antral mucosa ROS in patients with GU, DU or GU combined with DU (GU/DU) remains to be investigated. In this study, our aim was to determine the ROS by chemiluminescence (CL) assay of gastric antral mucosa in patients with peptic ulcer disease. Methods: Forty-five patients with GU, DU, or GU/DU (n=18, n=18, and n=9, respectively) were enrolled in this study. The diagnosis of peptic ulcer was based on the endoscopy presentation. Gastric mucosa specimens obtained from the gastric antrum for detection of O2(superscript -) or H2O2 activity were determined by CL assay. Protein concentrations in the tissue homogenates were determined by Bio-Rad protein assay. Results: There was no significant difference in amounts of O2(superscript -), H2O2, protein corrected O2 or H2O2 amounts among patients with GU, DU, or GU/DU. Results of CL assay revealed a significant difference in the amount of gastric antral mucosa H2O2 in peptic ulcer patients with H. pylori vs. those without H. pylori infection (H2O2 (counts/sec), 1512.67±747.97 vs. 342.39±205.40, p=0.045; H2O2 (counts/sec•μg), 43.79±22.30, 6.63±3.59, p=0.031). Conclusion: The role of H. pylori in the generation of ROS in gastric mucosa has been confirmed. In this study, we demonstrated that H. pylori increased H2O2 in gastric antral mucosa and there was no difference in gastric antral mucosa ROS between patients with GU, DU, and GU/DU.