Abstract
The formation of bacterial biofilm is a major challenge in clinical applications. The main aim of this study is to describe the synthesis, characterization and biocidal potential of zinc oxide nanoparticles (NPs) against bacterial strain Pseudomonas aeruginosa. These nanoparticles were synthesized via soft chemical solution process in a very short time and their structural properties have been investigated in detail by using X-ray diffraction and transmission electron microscopy measurements. In this work, the potential of synthesized ZnO-NPs (∼10–15 nm) has been assessed in-vitro inhibition of bacteria and the formation of their biofilms was observed using the tissue culture plate assays. The crystal violet staining on biofilm formation and its optical density revealed the effect on biofilm inhibition. The NPs at a concentration of 100 µg/mL significantly inhibited the growth of bacteria and biofilm formation. The biofilm inhibition by ZnO-NPs was also confirmed via bio-transmission electron microscopy (Bio-TEM). The Bio-TEM analysis of ZnO-NPs treated bacteria confirmed the deformation and damage of cells. The bacterial growth in presence of NPs concluded the bactericidal ability of NPs in a concentration dependent manner. It has been speculated that the antibacterial activity of NPs as a surface coating material, could be a feasible approach for controlling the pathogens. Additionally, the obtained bacterial solution data is also in agreement with the results from statistical analytical methods.
Highlights
Increased resistance of bacteria against antibiotic medicines is a global health concern
The size of the grown nanoparticles was further analyzed with X-ray diffraction pattern (XRD) and it demonstrates the crystallinity of the prepared white powder under defined condition earlier
No other peak related to impurities was observed in the spectrum within the detection limit of the X-ray diffraction, which further confirms that the synthesized powder consists of almost pure ZnO nanoparticles
Summary
Increased resistance of bacteria against antibiotic medicines is a global health concern. The tetrapod like structures of ZnO synthesized by flame transport synthesis process capacity to block the entry and spread of HSV-2 virus into target cells and have ability to neutralize HSV-2 virions [11,12,13]. Towards this direction, several instrumentation and methods have been applied to observe the accuracy and reliability of bacterial strain solution result such as inductively coupled plasma atomic emission spectrometery (ICPAES), photoluminescence (PL) spectroscopy, atomic absorption spectrophotometer (AAS), X-ray fluorescence spectrometery (EDX). There is a growing demand to determine the most appropriate and exact analytical methods for statistical analytical regression analysis to monitor the used nanostructures
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