Reactive oxygen species generation and dispersant-dependent electron transfer through single-walled carbon nanotubes in water

Abstract

Although induction of oxidative stress is widely accepted as one of the major cytotoxic effects of carbon nanotubes (CNTs), there is no solid understanding of how biological redox reactions are affected and how reactive oxygen species (ROS) are generated by CNTs, especially when they are coated with various dispersing agents. In this study, we investigated electron transfer from biological reducing agents through nonfunctionalized single-walled carbon nanotubes (SWCNTs) to molecular oxygen, generating ROS in the process. Electron transfer rates in the colloidal SWCNT suspensions depended on the dispersant used to stabilize them, with six dispersants examined. Oxidation of both nicotinamide adenine dinucleotide (NADH) and dithiothreitol was catalyzed by SWCNTs coated with either cetyltrimethylammonium bromide (CTAB) or Suwannee River natural organic matter (SRNOM). SWCNTs coated with other types of surfactants showed only slight effect. In the presence of NADH or dithiothreitol, generation of ROS also was dispersant-dependent, with CTAB- and SRNOM-coated SWCNTs generating significant amounts of superoxide anion and hydrogen peroxide. In systems containing xanthine and xanthine oxidase, accumulated charge on the SWCNTs appeared to be transferred to superoxide anion, resulting in indirect disproportionation of superoxide anion, forming more hydrogen peroxide.