Reactive oxygen species and other biochemical and morphological biomarkers in the gills and kidneys of the Neotropical freshwater fish, Prochilodus lineatus, exposed to titanium dioxide (TiO2) nanoparticles.

Abstract

This study investigated the action of titanium dioxide nanoparticles (TiO2-NPs), on the gills and kidneys of Neotropical freshwater fish, Prochilodus lineatus, with emphasis on reactive oxygen species (ROS) production, antioxidant responses, and morphological changes. Fish were exposed to 1, 5, 10, and 50mgL-1 nominal TiO2-NPs suspended into water for 2 or 14days. In gills, ROS decreased and glutathione (GSH) increased after 2days, while ROS and GSH increased and superoxide dismutase activity decreased after 14days. In kidneys, GSH and lipoperoxidation increased after 2days and catalase activity decreased after 14days. Common histopathologies in gills were epithelium hyperplasia, cellular hypertrophy, proliferation of mitochondria-rich cells (MRC), and lamellar stasis; in kidneys, there were cellular and nuclear hypertrophy, focal tubule degeneration, necrosis, and melanomacrophage (MM) proliferation. Although environmentally unlikely, high-dose exposures clarified biological effects of TiO2-NPs, such as ROS formation and MRC responses in the gills, which may impair ionic balance. It was also found that MM are likely responsible for eliminating NPs in the kidney. These findings will help to regulate TiO2-NP disposal, but longer-term studies are still needed.