Reactive oxygen probes - a broad range of colors with easier labeling and compatibility with fixation: novel CellROX® reagents from Molecular Probes® (P3295)

Abstract

Abstract A natural consequence of aerobic respiration is the generation of highly toxic radicals called reactive oxygen species (ROS). ROS have been implicated in various human pathologies. Although fluorogenic probes such as Aminophenyl fluorescein (APF) and hydroxyphenyl fluorescein (HPF), and 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) have been widely used to detect ROS using flow cytometry and imaging platforms, all are excitable by the 488nm laser and emit in the fluorescein channel, and require that labeling occur in a protein-free buffer. In contrast, the novel CellROX® ROS detection reagents from Molecular Probes® offer increased flexibility for multiplex experiments, with fluorescence emission in the fluorescein, PE, or APC channels for CellROX® Green, Orange, and Deep Red. In addition, the CellROX® reagents offer increased ease of use with the ability to label cells in complete growth media, have increased photostability as compared to H2DCFDA, and are compatible with fixation (CellROX® Green and Deep Red). The CellROX® ROS detection reagents are bright and stable ROS sensors that offer significant advantages over existing ROS sensors because they are compatible with labeling in different media and can be used with fixatives. For Research Use Only. Not for use in diagnostic procedures.