Abstract
Problem statement: Tuberculosis (TB) remains one of the world's major health problems. To evaluate in vivo indices of cellular sensitization, antigen-induced Reactive Nitrogen Intermediates (RNI) responses by blood mononuclear cells from Mycobacterium bovis BCG-infected goats supplemented with 1, 25 dyhidroxyvitamin D3 [1, 25-(OH)2D3]. Approach: An experimental, longitudinal and comparative study was planned. Twelve animals of goat cattle 20-to 24-month-old sannen selected themselves. Five samplings were made, previous to the inoculation (zero d), 3, 7, 14 and 21 d after applying the treatments. The mononuclear cells by the Ficoll-hypaque method were obtained. The RNI, nitrites and nitrates (NO2- and NO3-) were quantified by Enzyme-Linked Immunosorbent Assay (ELISA). Results: The treatment with the 1, 25(OH)2D3 stimulated the NO3- synthesis indicating, that by itself it is a good modulador of the micobacterial replication and in the treatment with M. bovis-BCG vaccine increased as a result to the treatment with 1,25(OH)2D3. The exhibition to M. bovis-BCG vaccine with the treatment with 1, 25(OH)2D3 was able to increase answer NO3- in exposed animals. Conclusion: The 1, 25(OH)2D3 stimulated in vivo the production of RNI in goats exposed to M. bovis BCG vaccine.
Highlights
Public health to control their dissemination[2,3], the majority of such infections remain clinically latent[4], Tuberculosis (TB) remains one of the world’s the pathogeneicity include its ability to resist the harsh major health problems and the leading cause of death from a single infectious agent called Mycobacterium tuberculosis, the primary causative agent[1]
Control animals NO3− concentrations were between 26.06-39.23 μ M−l, the supplemented treatment with M. bovis Bacillus Calmette-Guerin (BCG) vaccine were 17.77-64.27 μ M−l, Fig. 1: Average of minimum squares and standard errors of the concentration of nitrites in cells culture when applying different treatments increased the answer of the immune system in goats
Control animals NO2− concentrations were between 7.9-18.64 μ M−l, the culture when applying different treatments increased the answer of the immune system in goats
Summary
Public health to control their dissemination[2,3], the majority of such infections remain clinically latent[4], Tuberculosis (TB) remains one of the world’s the pathogeneicity include its ability to resist the harsh major health problems and the leading cause of death from a single infectious agent called Mycobacterium tuberculosis, the primary causative agent[1]. Designated M. tuberculosis infects a third of the world- One essential component of tubercular host defense wide population to the year, in spite of the efforts of includes Nitric Oxide (NO). Animal & Vet. Sci., 4 (1): 6-13, 2009 determined that there are two essential components of high level of immunity and that the sensitization of very tubercular host defense include NO and Tumor young animals to antigens of environmental. INOS macrophages are among the first cells in innate resistance to intracellular microbial pathogens, they have been determining that the cytokines gammasynthesis is essential for maintaining stationary-level Interferon (IFN-γ), Granulocyte-Macrophage Colonyinfection[10], has been investigated iNOS presence in Stimulating Factor (GM-CSF) and TNF-α activate biopsies exhibiting dermal nerves from patients with macrophages to resist the growth of intracellular untreated leprosy[11]. NO Reactive Oxigen Intermediates (ROI)[8,21], responses play an important role in organism and host proinflammatory cytokines such as interleukin (IL-12)
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