Abstract
3,4-Dihydroxybenzoic acid, commonly known as protocatechuic acid, is a naturally occurring phenolic compound, being the active component of many medicinal and edible plants. The in vitro and in vivo studies of protocatechuic acid conclude that it possesses many pharmacological properties. Protocatechuic acid, present in waste streams of food processing industries, is considered a phenolic pollutant. Owing to its bactericidal properties and in order to maintain the standards of disposal, its removal from the waste streams is necessary. Protocatechuic acid finds applications also in bioplastics, polymers, and also bio-based active films to improve food preservation. Its direct extraction from plant secondary metabolites possesses many difficulties. Recently reports of protocatechuic acid production by several Bacillus species are present in literature. For the retrieval/removal of protocatechuic acid from aqueous streams, methods like adsorption, O3/UV or H2O2/UV, and microbial degradation are in practice. For the retrieval of carboxylic acid from fermentation broths and aqueous streams, reactive extraction by the use of specific extractants has been found to be a most suitable method owing to its several advantages. The present paper is focused on the separation of protocatechuic acid by reactive extraction as a promising approach. The parameters needed for the design such as distribution coefficient, water co-extraction, physical and chemical extraction, effect of initial acid concentration, diluents, extractant, and extractant concentration have been discussed.
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