Abstract
AbstractThe separation of intracellular enzymes from disintegrated microbial cells is one of the most crucial steps in enzyme production. One way to overcome this problem is the application of physical extraction in polymer/salt based aqueous phase systems. An alternative, more recent approach being still under investigation is reactive extraction — called affinity partitioning/extraction in biotechnology — carried out in aqueous two‐polymer phase systems.In the intended contribution affinity extraction will be discussed with regard to the— physico‐chemical basis and general potential• parameters influencing affinity partitioning• modeling• selectivity• product yield• ways to improve performances• “product elution”/product separation from the ligand• limitations• comparison with physical extraction and— technical aspects• scale‐up• ligand recycling• continuous processing• economicsThe presentation will be based on recent literature data and several process examples being presently studied in the author's laboratories.
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