Abstract
Abstract The reaction of normal human and animal sera with heat-aggregated serum γ globulin (FII) was studied by means of double diffusion precipitation tests in agarose gel. Best results were obtained using agarose dissolved in a low molar salt solution (0.075 M NaCl). Precipitation lines were obtained, and in some instances improved, in the presence of EDTA. The double diffusion tests in agarose gel could be employed as a simple and convenient analytic tool for identification of the precipitin as the Clq component of complement. The reaction of Clq of human and animal origin was stronger with aggregated rat FII than with aggregated human FII. No reactions were obtained with bovine, sheep or rabbit FII. The anticomplementary activity of FII preparations in a hemolytic system paralleled their activity in the precipitation tests. Eluates containing Clq were prepared from precipitates formed in the reaction between human serum and rat FII in the presence of EDTA. Antisera specific for human Clq were produced in rats by injection of such precipitates.
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