Reaction patterns of five strains of infectious pancreatic necrosis virus in enzyme-linked immunosorbent assay.

Abstract

Comparison of reaction patterns among the five strains of infectious pancreatic necrosis virus (IPNV) and an infectious bursal disease virus (IBDV) was done by using enzyme-linked immunosorbent assay (ELISA) with antiserum against purified VR299 strain of IPNV. Five strains of IPNV were divided into three groups by the reaction pattern: i) VR299 strain, ii) Buhl and Powder Mill strains, and iii) d'Honnincthun and Bonnamy strains. IBDV could not be detected by ELISA using the anti-IPNV serum. Molecular weight comparison of virion proteins revealed that size of β proteins of IPNV was unique in three groups of IPNV. Antigenic relationships among IPNV strains examined by immunodiffusion (ID) showed that Buhl and Powder Mill strains were similar to VR299 strain, but d'Honnincthun and Bonnamy strains were distinct from other three strains of IPNV. No cross reaction was observed between IBDV and IPNV-VR299 strain by ID. These results indicate that ELISA can be used to distinguish antigenic differences among IPNV strains and to differentiate IPNV from antigenically unrelated virus such as IBDV which is classified in the same virus family as IPNV.