Reaction of sperm with egg‐derived hydrogen peroxide helps prevent polyspermy during fertilization in the sea urchin

Abstract

AbstractRecent evidence suggests roles for egg derived hydrogen peroxide (H2O2) and ovoperoxidase (secreted by cortical granules) in both fertilization envelope hardening and the block to polyspermy in sea urchins. Strongylocentrotus purpuratus eggs were found to release H2O2 during the cortical reaction at fertilization. Treatment of sperm with equivalent concentrations of H2O2 resulted in a rapid loss of sperm fertilizing ability. Attempts were made to induce polyspermy by utilizing ovoperoxidase inhibitors at concentrations known to inhibit fertilization envelope hardening. Eggs fertilized in phenylhydrazine became polyspermic, while 3‐amino‐1,2,4‐triazole‐treated eggs did not. These data suggested that a sperm peroxidase might be involved in preventing polyspermy. This hypothesis was tested by the addition of phenylhydrazine or 3‐amino‐1,2,4‐trizaole to H2O2‐treated sperm. Phenylhydrazine acted to protect sperm fertility from H2O2, while 3‐amino‐1,2,4‐triazole increased the adverse effect of H2O2. Simultaneous addition of both inhibitors to sperm incubated in H2O2 gave an intermediate value of sperm fertility. These data indicate that (1) H2O2 generated by sea urchin eggs during the cortical reaction at fertilization is used for two separate processes, fertilization envelope hardening and the prevention of polyspermy; (2) ovoperoxidase is probably not involved in preventing polyspermy; and (3) egg‐derived H2O2 reacts directly with sperm enzymes to prevent polyspermy. The phenylhydrazine‐sensitive enzyme in the sperm is probably a peroxidase that acts to inactivate sperm, while the 3‐amino‐1,2,4‐triazolesensitive enzyme is probably a catalase which protects sperm from H2O2. This hypothesis is consistent with model experiments on horseradish peroxidase and bovine liver catalase.