Reaction of advanced lines of triticale to karnal bunt (Tilletia indica)

Abstract

Twenty advanced lines of triticale were evaluated for resistance to karnal bunt during the 2014-2015 crop season, at the Norman E. Borlaug Experimental Station. The sowing dates were November 19 and 29, 2014. One mL of an allantoid sporidial suspension (10,000/mL) was injected during the boot stage of ten spikes per line. A mist-irrigation system was used to provide high humidity in the experimental area. Harvesting and threshing were done manually, and the counting of infected and healthy grains was perfomed by visual inspection. The range of infection for the first sowing date was 0 - 2.4%, with an average of 0.3, and for the second 0 - 1.4%, with an average of 0.4. Five lines did not show any infected grains in both dates and the rest was in the 0.1-2.5% infection category. The average of infection of the susceptible check during the period of inoculation of this group of lines was 86.6%. The highest average percentage of infection of both dates were shown by lines: TURACO/CENT.SARDEV/7/LIRON_2/5/DISB5/3/SPHD/ PVN//YOGUI_6/4/KER_3/6/BULL_10/MANATI_1/8/LIRON_2/5/DISB5/3/SPHD/PVN// YOGUI_6/4/KER_3/6/BULL_10/MANATI_1/9/BICEN (CTSS08Y00035S-099Y-026M-19Y-099M-2Y-2BMX-4Y) with 1.6 and POPP1_2/CAAL//THELIN#2/5/PRESTO//2*TESMO_1/ MUSX603/4/ARDI_1/TOPO1419//ERIZO_9/3/SUSI_2/6/ARDI/GNU//2*FAHAD_1/4/BULL_ 10/MANATI_1/3/ELK54/ BUF_2//NIMIR_3 with 1.3%. These lines also showed the highest percentage of infection in the first date with 1.8 and 2.4%, respectively.