Abstract
BackgroundREAC technology (acronym for Radio Electric Asymmetric Conveyor) is a technology platform for neuro and bio modulation. It has already proven to optimize the ions fluxes at the molecular level and the molecular mechanisms driving cellular asymmetry and polarization.MethodsThis study was designed to verify whether this technology could extend spermatozoa life-span during liquid storage, while preserving their functions, DNA integrity and oxidative status. At 0, 24, 48, and 72 h. of storage at 4 °C, a battery of analyses was performed to assess spermatozoa viability, motility parameters, acrosome status, and DNA integrity during REAC treatment. Spermatozoa oxidative status was assessed by determining lipid peroxidation, the activity of superoxide dismutase (SOD), and the total antioxidant capacity.ResultsDuring liquid storage REAC treated spermatozoa, while not showing an increased viability nor motility compared to untreated ones, had a higher acrosome (p > 0.001) and DNA integrity (p > 0.01). Moreover, the analysis of the oxidative status indicated that the mean activity of the intracellular superoxide dismutase (SOD) was significantly higher in REAC treated spermatozoa compared to untreated controls (p < 0.05), while the intracellular concentration of malondialdehyde (MDA), an end product of lipid peroxidation, at the end of the REAC treatment was higher in untreated controls (p > 0.05). The REAC efficacy on spermatozoa oxidative status was also evidenced by the higher trolox equivalent antioxidant capacity (TEAC) found in both the cellular extract (p < 0.05) and the storage media of REAC treated spermatozoa compared to untreated controls (p < 0.0001).ConclusionThe present study demonstrated that REAC treatment during liquid storage preserves spermatozoa acrosome membrane and DNA integrity, likely due to the enhancement of sperm antioxidant defenses. These results open new perspective about the extending of spermatozoa functions in vitro and the clinical management of male infertility.
Highlights
REAC technology is a technology platform for neuro and bio modulation
The present study demonstrated that REAC treatment, while not affecting sperm viability and motility during refrigeration, preserves spermatozoa acrosome membrane and DNA integrity
This effect is likely to be mediated by the enhancement of sperm antioxidant defenses after REAC treatment, as evidenced by the higher superoxide dismutase (SOD) activity, the lower MDA intracellular concentration at the end of the treatment, and the higher antioxidant capacity of the cell extract and the culture medium
Summary
REAC technology (acronym for Radio Electric Asymmetric Conveyor) is a technology platform for neuro and bio modulation. It has already proven to optimize the ions fluxes at the molecular level and the molecular mechanisms driving cellular asymmetry and polarization. Radio Electric Asymmetric Conveyer Technology for therapeutic use (REAC) is a recent technology whose aim is to optimize the ion fluxes at the molecular level, and to concentrate all the micro currents produced by these ion fluxes through the asymmetric conveyer-probe to optimize the molecular mechanisms driving cellular asymmetry and polarization. Development, survival, and effectiveness of sperm are closely linked to the phenomena of cell polarity. On these bases, we checked whether REAC technology is effective in extending and maintaining sperm functions and DNA integrity during liquid storage at 4 °C. During sperm liquid storage, we observe a deterioration of sperm functions followed by DNA damage [11]
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