Abstract
The ability of a single injection of estradiol benzoate (EB, 20 μg) to induce proestrus-like surges of luteinizing hormone (LH) on the day following EB administration was tested in long term ovariectomized rats which had been pretreated with EB (20 μg) and/or progesterone (P, 2.5 mg). LH afternoon surges could be evoked only in those animals which had previously received both ovarian hormones, EB and P. Pretreatment with a single hormone was ineffective. Moreover, in rats preinjected only with P the LH surge was shifted from afternoon to morning hours.
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