Re-Epithelialization Appraisal of Skin Wound in a Porcine Model Using a Salmon-Gelatin Based Biomaterial as Wound Dressing.

Cristian A Acevedo,Nicole Orellana,Patricio Morales,Javier Enrione,Elizabeth Sánchez,Donald I Brown,Yusser Olguín

doi:10.3390/pharmaceutics11050196

Abstract

The design of new functional materials for skin tissue engineering is an area of constant research. In this work, a novel wound-dressing biomaterial with a porous structure, previously formulated using salmon-gelatin as main component (called salmon-gelatin biomaterial (SGB)), was tested in vivo using pigs as skin wound models. Four weeks after cutaneous excision and implantation in the animals, the healing process did not show apparent symptoms of inflammation or infection. Interestingly, the temporal evolution of wound size from 100% to around 10% would indicate a faster recovery when SGB was compared against a commercial control. Histological analysis established that wounds treated with SGB presented similar healing and epithelialization profiles with respect to the commercial control. Moreover, vascularized granulation tissue and epithelialization stages were clearly identified, indicating a proliferation phase. These results showed that SGB formulation allows cell viability to be maintained. The latter foresees the development of therapeutic alternatives for skin repair based on SGB fabricated using low cost production protocols.

Highlights

The pursuing of efficient, sustainable and safe alternative biomaterials is an area of constant research [1,2]
Due of the risk of transmission to humans of certain diseases such as bovine spongiform encephalopathy and foot-and-mouth disease, the gelatin extracted from fish offers an alternative in terms of biosecurity, which has led to a growing interest in research and development of materials formulated from this source [7,8,9]
We studied the use of a wound-dressing biomaterial based on salmon-gelatin, with regular porous microstructure, on the healing process and re-epithelialization in a porcine skin model

Summary

Introduction

The pursuing of efficient, sustainable and safe alternative biomaterials is an area of constant research [1,2]. Two types of gelatin have been studied for the conformation of biomaterials for tissue regeneration, originating from mammals and from fish [6,7]. In this regard, due of the risk of transmission to humans of certain diseases such as bovine spongiform encephalopathy and foot-and-mouth disease (both from mammalian sources), the gelatin extracted from fish offers an alternative in terms of biosecurity, which has led to a growing interest in research and development of materials formulated from this source [7,8,9]. Gelatin from cold-adapted species such as salmon (Salmon salar) offers the advantage for composite fabrication due to its low viscosity at ambient temperature due to is smaller average molecular weight and lower gelling temperature compared to those from mammals

Methods

Discussion

Conclusion