RC-IAL cell line: sensitivity of rubella virus grow.

Cristina A Figueiredo,Ana Ms Afonso,Maria I Oliveira,Eliane Moreira,Aurea S Cruz,Suely P Curti,Luís Florêncio De Salles-Gomes

doi:10.1590/s0034-89102000000400007

Cristina A Figueiredo, Ana Ms Afonso + Show 5 more

Open Access

https://doi.org/10.1590/s0034-89102000000400007

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Abstract

The rapid growth of the rubella virus in RC-IAL2 with development of cytopathic effect, in response to rubella virus infection, is described. For purposes of comparison, the rubella virus RA-27/3 strain was titered simultaneously in the RC-IAL, Vero, SIRC and RK13 cell lines. Rubella virus RA-27/3 strain are inoculated in the RC-IAL cell line (rabbit Kidney, Institute Adolfo Lutz). Plates containing 1.5x10(5) cells/ml of RC-IAL line were inoculated with 0.1ml s RA-27/3 strain virus containing 1x 10(4)TCID50/0.1ml. A 25% cytopathic effect was observed after 48 hours and 100% after 96 hours. The results obtained were compared to those observed with the SIRC, Vero and RK13 cell lines. Rubella virus was detected by immunohistochemistry. With the results, it was possible to conclude that the RC-IAL cell line is a very good substrate for culturing rubella virus. The cells inoculated with rubella virus were examined by phase contrast microscopy and showed the characteristic rounded, bipolar and multipolar cells. The CPE in RC-IAL was observed in the first 48 hours and the curve of the increased infectivity was practically the same as observed in other cell lines. These findings are important since this is one the few cell lines described in the literature with a cytopathic effect. So it can be used for antigen preparation and serological testing for the diagnosis of specific rubella antibodies.

Highlights

Rubella virus (RV) replicates in a variety of cell culture systems, primary cells and continuous cell lines.[1,3,4,8] Usually, rabbit Kidney (RK13) and rabbit cornea (SIRC) are used for rubella virus isolation.In the past, RV has been identified by interference techniques in primate African green monkey kidney cells (AGMK).[9,10] As these cells are rarely available, the continuous kidney African green monkey (Vero) cell line is a more suitable alternative
The cells inoculated with rubella virus were examined by phase-contrast microscopy and revealed an increased cellular refractability and rounding of the infected cells
The cytophatic effect (CPE) was found to decrease significantly if infected cultures had more than one medium replacement during the observation period

Summary

Introduction

Rubella virus (RV) replicates in a variety of cell culture systems, primary cells and continuous cell lines.[1,3,4,8] Usually, rabbit Kidney (RK13) and rabbit cornea (SIRC) are used for rubella virus isolation. RV has been identified by interference techniques in primate African green monkey kidney cells (AGMK).[9,10] As these cells are rarely available, the continuous kidney African green monkey (Vero) cell line is a more suitable alternative. As Vero cells do not produce interferon, RV can replicate more rapidly and reach higher titers in these cells. Baby Hamster kidneys (BHK-21) and Vero cell cultures are used extensively in the production of high titers of RV required as antigens in serological tests.[4]

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