Rauvolfia vomitoria and Gongronema latifolium stimulate cortical cell proliferations

Abstract

Introduction: Rauwolfia vomitoria (RV) and Gongronema latifolium (Gl) are herbs with closely related and diverse medicinal properties. The combination of both plants is reported to have the potentials for brain functions and structure protection. Aim: This study therefore investigated the interaction of these herbs on the histomorphology of the cerebral cortex of mice. Materials and Methods: 24 male Wistar mice of body weight 15-26 g were divided into 4 groups. The mice were administered respectively, 0.5 mL of Tween 20, 150 mg/kg of R. vomitoria , 200 mg/kg of G. latifolium , and a combination of 150 mg/kg of R. vomitoria and 200 mg/kg of G. latifolium (RV+GL), orally, and daily for seven days. On day 8, the animals were sacrificed and their brains preserved, and the cerebral cortices were excised for routine histology. Cellular densities were quantified using ImageJ™. Results: All the groups gained body weight, which was however lower in the test groups compared with the control group. No difference was observed in whole brain weight in all the experimental groups, while histomorphological studies of the cerebral cortex showed higher cellular density and smaller cellular sizes in the RV, GL and RV+GL groups. The RV+GL group also showed slightly larger cells in the cortical plate compared with the control group. The mean cellular population of sections of the cerebral cortex were also higher in test groups RV and GL, but not the RV+GL group. Conclusion: This study showed that R. vomitoria root bark and G. latifolium leaf extracts either singly or in combination may stimulate cellular proliferation at the given dose, which may serve a protective or deleterious role.