Abstract
Diploid and polyploid nuclei have been isolated from the livers of 3–4-month-old rats. The extent of contamination of each fraction with the other fraction being probably not above 10%, as shown by microscopic counting and analyses of DNA per nucleus. (The average amount of DNA per diploid nucleus was 5.5 pg and per polyploid nucleus was 12.8 pg.) No differences could be established in the ratios of the globulin fraction, total histone, or residual proteins to DNA for the two classes of nuclei. The disc-gel electrophoretic patterns and analyses of histones for amino acids were also the same for diploid and polyploid nuclei.
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